DETAILS – Pfizer Vaccine Becomes DNA in The Human Liver Cells Huh7. (In-vitro Swedish Study) | DrBeen Medical Lectures | Mon, Feb 28, 2022 (Video with References)


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Drbeen Medical Lectures
~ 1 hr

An accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. 

It is reverse transcribed by LINE-1 proteins of the cells. They are not sure if it gets integrated in our DNA or not. Let’s review the details of their method.



00:00 introduction 
00:45 Link to the summary 
01:00 Review of the reference material 
02:19 Cell preparation for the vaccine  
03:35 Vaccine addition to the cells  
06:00 RT-PCR for find RNA and then DNA  
13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA
18:27 Measuring the volume of proteins in the cytosol and the nucleus  
22:35 DNA purification and verification  
28:41 Results  
28:42 LNP enters the liver cells efficiently  
29:33 LINE-1 gene is expressed  
30:46 ORF-1 found in cytosol and the nucleus  
36:01 DNA from the vaccine found in the cell  
43:30 Questions not answered
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URL list from Friday, Feb. 25, 2022
CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML

Frontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics,experimental%20substitute%20for%20primary%20hepatocytes


URL list from Monday, Feb. 28, 2022

CIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML

LINE-1 protein localization and functional dynamics during the cell cycle | eLife

Time evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv

Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues – PubMed

No evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues – PMC

Overview of Reverse Transcription – Retroviruses – NCBI Bookshelf

Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology

Chimeric RNA – Wikipedia

Benchtop Automation for Immunohistochemistry (IHC), ISH & Special Stains (SS) – NanoMtrx 300 – YouTube

Schrödinger’s cat – Wikipedia


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